Methods Northern blot, MTT determination,

and 3H thymidine incorporation were used to investigate the effects of antisense TGF β1 gene on osteosarcoma. Results The proliferation of osteosarcoma cells transfected by antisense TGF β1 gene was suppressed markedly, and adriamycin sensitivity was significantly increased. Conclusion Blockage of osteosarcoma cells TGF β1 autocrine loop inhibits cell proliferation PR 957 and enhances chemother-apy sensitivity.
Background This study was designed to investigate changes in mRNA levels of transforming growth factor-β(TGF-β), collagen Ⅰ, and collagen Ⅲ in autogenous vein grafts. Methods Twenty-four New Zealand rabbits were randomly divided into 4 groups with 6 rabbits each. The external jugular veins of the New Zealand rabbits were harvested and grafted Temsirolimus数据表 into the ipsilateral carotid artery. All rabbits were fed with a standard diet. After the operation, the rabbits were sacrificed at 1, 2, 3, or 4 weeks. TGF-β, collagen Ⅰ, and collagen

Ⅲ mRNA levels in the venous grafts were measured by semiquantitative methods at every time point. The contralateral external jugular veins were also harvested and analyzed as controls. Glyceraldehyde-3-phosphate dehydrogenase was used as an internal standard to normalize all samples for potential variations in mRNA

content. In order to observe the expression of TGF-β protein, immunohistochemical SABC methods were used. Results One week postoperation, the mRNA level of TGF-β was upregulated to 1.73±0.19 in the vein graft and 1.21±0.16 in the control vein (P
目的　探讨TGF – β1介导的Smad信号通路对人腹膜间皮细胞 (HPMCs)细胞外基质 (ECM)的调控机制。方法　原代培养的第三代HPMCs分成对照组与 5ng/mlTGF – β1刺激组 ,采用免疫组织化学染色和Western印迹法观察细胞内磷酸化Smad2 / 3(p -Smad2 / 3)的表达以及在细胞内的迁移 ,Western印迹、ELISA和RT 哪里 -PCR法观察Smad7、结缔组织生长因子 (CTGF)、α-平滑肌肌动蛋白 (α-SMA)、纤溶酶原激活物抑制剂 -1(PAI- 1)、纤维连接蛋白 (FN)和I型胶原 (COL1)的mRNA及蛋白表达。结果　①对照组细胞内几乎不表达p -Smad2 / 3,在刺激后 15min蛋白表达增加 ,主要分散在细胞质中 ,1h达高峰 ,主要集中在细胞核及周边 ,2h明显回落 ,又分散至细胞质中 ;②刺激组细胞内Smad7、CTGF、α -SMA和COL1的蛋白表达与对照组比均增加 ,其中Smad7、CTGF和α -SMA在 4 8h最明显 ,COL1呈时间依从性 ;刺激组上清液PAI – 1的蛋白含量与对照组比均增加 ,其中 2 4h最高 ,FN呈时间依从性 ;刺激组Smad7的mRNA表达与对照组比呈时间依从性增加 ,CTGF、α -SMA、PAI- 1、FN和COL1均增加 ,其中CTGF和α-SMA在 4 8h最高 ,PAI- 1在 2 4h最高 ,FN和COL1呈时间依从性。结论　TGF – β1能特异性激活HPMCs内Smad信号通路 ,并可通过诱导该通路下游靶基因Sm
TGF-β信号的转导与纤维化疾病的发生及癌细胞的侵入和转移高度相关,而TGF-β信号通路中的重要节点ALK5是治疗这些疾病的理想靶标。本文针对31个新型咪唑[2,1-b][1,3,4]噻二唑类ALK5抑制剂,分别运用Co MFA和Co MSIA两种经典方法进行了3D-QSAR研究,并获得了预测能力较优的两个模型(Co MFA:q2=0.